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bd permwash buffer goat antirabbit igg alexfluor 594 goat invitrogen a11012 immunostaining  (Thermo Fisher)


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    Thermo Fisher bd permwash buffer goat antirabbit igg alexfluor 594 goat invitrogen a11012 immunostaining
    Bd Permwash Buffer Goat Antirabbit Igg Alexfluor 594 Goat Invitrogen A11012 Immunostaining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permwash+buffer/pm38906885-425-198-207
    Average 86 stars, based on 1 article reviews
    bd permwash buffer goat antirabbit igg alexfluor 594 goat invitrogen a11012 immunostaining - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Staining:

    Article Title: PTPN2 Is a Critical Regulator of Ileal Paneth Cell Viability and Function in Mice
    Article Snippet: .. Cells then were fixed with the FoxP3 staining kit (eBioscience) according to the manufacturer’s instructions, stained with anti-FoxP3–Pacific Blue, anti–IFN-γ–PECy7, anti-IL17–APC, anti-TNFα–BV650, and anti-IL22–PE for 30 minutes, washed in PermWash buffer (eBioscience), samples were acquired on an LSRII cytometer (BD, Franklin Lakes, NJ), and analyzed using FlowJo (Tree Star, Inc, Ashland, OR). ..

    Article Title: TFAM-Dependent Mitochondrial Metabolism Is Required for Alveolar Macrophage Maintenance and Homeostasis.
    Article Snippet: Cells were washed twice with FACS buffer prior to fixation and permeabilization with the Foxp3 Transcription Factor Staining Buffer Set (eBioscience) for 1 h at room temperature in the dark. .. Cells were washed twice with PermWash buffer (eBioscience) and stained with Abs against Ki67 (eBioscience) and control Ig (BioLegend) in Perm Wash buffer for at least 30 min at room temperature in the dark. ..

    Article Title: A multi-enhancer hub at the Ets1 locus controls T cell differentiation and allergic inflammation through 3D genome topology
    Article Snippet: Cells were then either fixed for intracellular staining using the Foxp3 staining buffer (eBioscience) or were fixed with 2% PFA. .. For intracellular staining, fixed cells were washed with permwash buffer (eBioscience) and incubated with intracellular antibodies diluted in permwash buffer for 30 mins or left overnight. .. Cells were washed with permwash buffer and resuspended in FACS buffer with the addition of 123count eBeads (ThermoFischer Scientific, ref:01-1234-42) following manufacturer’s recommendations for cell counting.

    Article Title: PTPN2 Is a Critical Regulator of Ileal Paneth Cell Viability and Function in Mice.
    Article Snippet: .. Cells then were fixed with the FoxP3 staining kit (eBioscience) according to the manufacturer’s instructions, stained with anti-FoxP3–Pacific Blue, anti–IFN-g–PECy7, anti-IL17–APC, anti-TNFa–BV650, and anti-IL22–PE for 30 minutes, washed in PermWash buffer (eBioscience), samples were acquired on an LSRII cytometer (BD, Franklin Lakes, NJ), and analyzed using FlowJo (Tree Star, Inc, Ashland, OR). ..

    Article Title: Detection of rare autoreactive T cell subsets in patients with pemphigus vulgaris
    Article Snippet: For extracellular staining, the following monoclonal antibodies were used: CD4-AF 700 (RPA-T4, BD Biosciences, San Jose, CA, USA), CD8-PE-Cy7 (SK1), CXCR3-BV421 (G025H7), CD4-BV510 (RPA-T4), CD45RA-FITC (HI100), CD3-PerCP-Cy5.5 (SK7), CXCR5-PE (J252D4), CCR6-APC (G034E3; all BioLegend). .. For intracellular staining, cells were fixed and permeabilized using fixation/permeabilization buffer (eBioscience FOXP3/Transcription; Invitrogen, San Diego, CA) and stained intracellularly for 20 minutes at 4°C for functional readouts in permwash buffer (eBioscience FOXP3/Transcription; Invitrogen, San Diego, CA). .. For intracellular staining, the following monoclonal antibodies were used: CD154-BV421 (24-31), CD154-PE/Cyanine7 (24-31; both BioLegend), IL-4-BV786 (MP4-25D2), IFN-γ-FITC (B27), IL-17A-BV650 (N49-653), IL-21-PE (3A3-N2.1; all BD Biosciences, San Jose, CA, USA).

    Article Title: Quantitative control of Ets1 dosage by a multienhancer hub promotes Th1 cell differentiation and protects from allergic inflammation
    Article Snippet: Cells were then either fixed for intracellular staining using the Foxp3 staining buffer (eBioscience) or were fixed with 2% PFA. .. For intracellular staining, fixed cells were washed with permwash buffer (eBioscience) and incubated with intracellular antibodies diluted in permwash buffer for 30 mins or left overnight. .. Cells were washed with permwash buffer and resuspended in FACS buffer with the addition of 123count eBeads (ThermoFischer Scientific, ref:01–1234-42) following manufacturer’s recommendations for cell counting.

    Cytometry:

    Article Title: PTPN2 Is a Critical Regulator of Ileal Paneth Cell Viability and Function in Mice
    Article Snippet: .. Cells then were fixed with the FoxP3 staining kit (eBioscience) according to the manufacturer’s instructions, stained with anti-FoxP3–Pacific Blue, anti–IFN-γ–PECy7, anti-IL17–APC, anti-TNFα–BV650, and anti-IL22–PE for 30 minutes, washed in PermWash buffer (eBioscience), samples were acquired on an LSRII cytometer (BD, Franklin Lakes, NJ), and analyzed using FlowJo (Tree Star, Inc, Ashland, OR). ..

    Article Title: PTPN2 Is a Critical Regulator of Ileal Paneth Cell Viability and Function in Mice.
    Article Snippet: .. Cells then were fixed with the FoxP3 staining kit (eBioscience) according to the manufacturer’s instructions, stained with anti-FoxP3–Pacific Blue, anti–IFN-g–PECy7, anti-IL17–APC, anti-TNFa–BV650, and anti-IL22–PE for 30 minutes, washed in PermWash buffer (eBioscience), samples were acquired on an LSRII cytometer (BD, Franklin Lakes, NJ), and analyzed using FlowJo (Tree Star, Inc, Ashland, OR). ..

    Control:

    Article Title: TFAM-Dependent Mitochondrial Metabolism Is Required for Alveolar Macrophage Maintenance and Homeostasis.
    Article Snippet: Cells were washed twice with FACS buffer prior to fixation and permeabilization with the Foxp3 Transcription Factor Staining Buffer Set (eBioscience) for 1 h at room temperature in the dark. .. Cells were washed twice with PermWash buffer (eBioscience) and stained with Abs against Ki67 (eBioscience) and control Ig (BioLegend) in Perm Wash buffer for at least 30 min at room temperature in the dark. ..

    Incubation:

    Article Title: A multi-enhancer hub at the Ets1 locus controls T cell differentiation and allergic inflammation through 3D genome topology
    Article Snippet: Cells were then either fixed for intracellular staining using the Foxp3 staining buffer (eBioscience) or were fixed with 2% PFA. .. For intracellular staining, fixed cells were washed with permwash buffer (eBioscience) and incubated with intracellular antibodies diluted in permwash buffer for 30 mins or left overnight. .. Cells were washed with permwash buffer and resuspended in FACS buffer with the addition of 123count eBeads (ThermoFischer Scientific, ref:01-1234-42) following manufacturer’s recommendations for cell counting.

    Article Title: Quantitative control of Ets1 dosage by a multienhancer hub promotes Th1 cell differentiation and protects from allergic inflammation
    Article Snippet: Cells were then either fixed for intracellular staining using the Foxp3 staining buffer (eBioscience) or were fixed with 2% PFA. .. For intracellular staining, fixed cells were washed with permwash buffer (eBioscience) and incubated with intracellular antibodies diluted in permwash buffer for 30 mins or left overnight. .. Cells were washed with permwash buffer and resuspended in FACS buffer with the addition of 123count eBeads (ThermoFischer Scientific, ref:01–1234-42) following manufacturer’s recommendations for cell counting.

    Functional Assay:

    Article Title: Detection of rare autoreactive T cell subsets in patients with pemphigus vulgaris
    Article Snippet: For extracellular staining, the following monoclonal antibodies were used: CD4-AF 700 (RPA-T4, BD Biosciences, San Jose, CA, USA), CD8-PE-Cy7 (SK1), CXCR3-BV421 (G025H7), CD4-BV510 (RPA-T4), CD45RA-FITC (HI100), CD3-PerCP-Cy5.5 (SK7), CXCR5-PE (J252D4), CCR6-APC (G034E3; all BioLegend). .. For intracellular staining, cells were fixed and permeabilized using fixation/permeabilization buffer (eBioscience FOXP3/Transcription; Invitrogen, San Diego, CA) and stained intracellularly for 20 minutes at 4°C for functional readouts in permwash buffer (eBioscience FOXP3/Transcription; Invitrogen, San Diego, CA). .. For intracellular staining, the following monoclonal antibodies were used: CD154-BV421 (24-31), CD154-PE/Cyanine7 (24-31; both BioLegend), IL-4-BV786 (MP4-25D2), IFN-γ-FITC (B27), IL-17A-BV650 (N49-653), IL-21-PE (3A3-N2.1; all BD Biosciences, San Jose, CA, USA).

    FACS:

    Article Title: Quantitative control of Ets1 dosage by a multi-enhancer hub promotes Th1 cell differentiation and protects from allergic inflammation.
    Article Snippet: In brief Multi-enhancer hubs are spatial clusters of enhancers present across numerous developmental programs.. Chandra, Yoon, Michieletto, et al. examine the functional relevance of multi-enhancer hubs in T cell biology and show that the multiplicity of enhancers interacting with the Ets1 gene is essential to control the precise dosage of Ets1 in response to cytokine cues, and the failure to do so can lead to allergic diseases.

    Cell Counting:

    Article Title: Quantitative control of Ets1 dosage by a multi-enhancer hub promotes Th1 cell differentiation and protects from allergic inflammation.
    Article Snippet: In brief Multi-enhancer hubs are spatial clusters of enhancers present across numerous developmental programs.. Chandra, Yoon, Michieletto, et al. examine the functional relevance of multi-enhancer hubs in T cell biology and show that the multiplicity of enhancers interacting with the Ets1 gene is essential to control the precise dosage of Ets1 in response to cytokine cues, and the failure to do so can lead to allergic diseases.



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    Image Search Results


    Journal: Nature Communications

    Article Title: ATM inhibition exploits checkpoint defects and ATM-dependent double strand break repair in TP53 -mutant glioblastoma

    doi: 10.1038/s41467-024-49316-8

    Figure Lengend Snippet:

    Article Snippet: For staining with anti-cleaved PARP, cells were treated in a similar fashion, except they were incubated with 1:200 dilution of anti-cleaved PARP (Asp214) for 1 h, washed twice with PermWash buffer (BD), and then incubated for 1 h with Alexafluor 488-conjugated anti-rabbit secondary for U251 or A172 cells and then resuspended in 2% FBS with propidium iodide and RNAse A.

    Techniques: Western Blot, Immunostaining